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Cytoadherence and apoptosis of <t> HLEC </t> and <t> hCMEC/D3 </t> mediated by P. falciparum infected red blood cell
Confluent Hlec And Hcmec/D3 Monolayers, supplied by Labtek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cytoadherence and apoptosis of HLEC and hCMEC/D3 mediated by P. falciparum infected red blood cell

Journal: BMC Infectious Diseases

Article Title: Comparison of apoptosis in human primary pulmonary endothelial cells and a brain microvascular endothelial cell line co-cultured with Plasmodium falciparum field isolates

doi: 10.1186/s12879-017-2552-0

Figure Lengend Snippet: Cytoadherence and apoptosis of HLEC and hCMEC/D3 mediated by P. falciparum infected red blood cell

Article Snippet: A suspension of 300 μl of mature pRBC at 6% parasitaemia and 2% haematocrit (approximately 4 × 10 6 schizonts for 63 × 10 6 uninfected RBC) in complete RPMI culture medium without bicarbonate was added to each well containing confluent HLEC and hCMEC/D3 monolayers from Labtek in duplicate, and incubated for 1 h at 37 °C.

Techniques: Infection

PRBC-mediated EC apoptosis. a ) PRBC-mediated EC apoptosis by direct contact with and without cytoadherence The ability of field isolates to induce EC apoptosis was the same for hCMEC/D3 (9 apoptogenic PRBCs) and HLEC (4 apoptogenic PRBCs) ( p > 0.05) and the mean optical density was also similar ( p > 0.05); b ) PRBC-mediated EC apoptosis by release of soluble factors. hCMEC/D3 were more susceptible to apoptosis mediated by soluble factors (11 apoptogenic PRBCs) than were HLEC (4 apoptogenic PRBCs) ( p < 0.05) but intensity (mean OD ratio) of this apoptosis was higher with hCMEC/D3 ( p < 0.01). P. falciparum field isolates trigger cerebral cells apoptosis than lung cells ( p < 0.01)

Journal: BMC Infectious Diseases

Article Title: Comparison of apoptosis in human primary pulmonary endothelial cells and a brain microvascular endothelial cell line co-cultured with Plasmodium falciparum field isolates

doi: 10.1186/s12879-017-2552-0

Figure Lengend Snippet: PRBC-mediated EC apoptosis. a ) PRBC-mediated EC apoptosis by direct contact with and without cytoadherence The ability of field isolates to induce EC apoptosis was the same for hCMEC/D3 (9 apoptogenic PRBCs) and HLEC (4 apoptogenic PRBCs) ( p > 0.05) and the mean optical density was also similar ( p > 0.05); b ) PRBC-mediated EC apoptosis by release of soluble factors. hCMEC/D3 were more susceptible to apoptosis mediated by soluble factors (11 apoptogenic PRBCs) than were HLEC (4 apoptogenic PRBCs) ( p < 0.05) but intensity (mean OD ratio) of this apoptosis was higher with hCMEC/D3 ( p < 0.01). P. falciparum field isolates trigger cerebral cells apoptosis than lung cells ( p < 0.01)

Article Snippet: A suspension of 300 μl of mature pRBC at 6% parasitaemia and 2% haematocrit (approximately 4 × 10 6 schizonts for 63 × 10 6 uninfected RBC) in complete RPMI culture medium without bicarbonate was added to each well containing confluent HLEC and hCMEC/D3 monolayers from Labtek in duplicate, and incubated for 1 h at 37 °C.

Techniques: